The family Parmeliaceae includes the largest diversity of foliose macrolichens, with over 71 genera and over 2,000 described species belonging to such iconic genera as Xanthoparmelia, Usnea, and Hypotrachyna. One genus, Cetrelia has been repeatedly shown to be monophyletic reinforcing its delimitation with phenotypic characters. Species of Cetrelia delimitated by chemical and morphological data have been largely supported by phylogenetic analysis of molecular data. While there is a robust, well-sampled global phylogeny for Cetrelia, it lacks sampling from Eastern North America (ENA) which is a region with important disjunct subpopulations of at least three species (C. chicitae, C. monachorum, and C. olivetorum). The focus of this study was to place the ENA subpopulations into the broader global Cetrelia phylogeny and confirm their taxonomic identities based on morphological and chemical characteristics. Targeted fieldwork was conducted by the first author in the Appalachian Mountains of North Carolina, Virginia, and West Virginia in 2022. Cetrelia specimens collected and sequenced for this study all were recovered in existing monophyletic or strongly supported groups when included with the global molecular dataset published by Mark et al (2019), and support three species (C. chicitae, C. monachorum, and C. olivetorum) being delimited by chemical and morphological characteristics in ENA. One species C. cetrarioides, also occurs in the region but was not successfully sequenced despite targeted attempts at new collections. Our study of C. cetrarioides suggests that morphological characters may not be enough to distinguish between it and C. monachorum in ENA and further molecular sequencing is needed to clarify this subpopulation as a chemotype of C. monachorum or phenotypic variation within C. cetrarioides.