A. africana is a medicinal and multipurpose tree that is under permanent pressure from its continuous exploitation for medicine, food and timber products.  In addition to this, the seeds are dormant and recalcitrant with a low rate of seed germination thus constraining propagation by conventional planting. There has been no literature documentation of any previous micro-propagation study on A. africana. Thus, this study is the first on callus induction and multiple shoot formation in Afzelia africana.    Cotyledonary node, hypocotyl and nodal explants excised from in vitro seedlings and cultured on Murashige and Skoog (MS) medium 1962 with different concentrations and combinations of plant growth regulators and phloroglucinol.  The experiment was carried out in two stages. The first evaluated the responsiveness of the different explants of A. africana in vitro while the second stage was done in 3 sets. The first set investigated the effect of plant growth regulators (benzyladenine BA, phloroglucinol Pg and Indole Acetic Acid IAA) interacting with age of cotyledonary node explants on shoot and callus initiation; the second investigated the effect of thiaduzuron TDZ, BA and Pg on shoot multiplication while  the last experiment investigated the effect of BA and Pg on shoot elongation. The results obtained showed that cotyledonary node among other explants responded best., MS media incorporated with 15mg/L BA, 15mg/L PG and 5mg/L TDZ favored shoot multiplication producing 10 shoots per explant. Also, MS media incorporated with 15mg/L BA, 15mg/L PG and 15mg/L IAA was best for callus initiation and MS media incorporated with 15mg/L BA and 15mg/L PG produced the longest shoot (8.65 cm).  This study has paved a path for the rapid regeneration of A. africana through in vitro propagation.