Soybean is an economic crop, but drought is considered one of the major setbacks in its production. Breeding programs have resulted in improved high yielding cultivars, but they are still susceptible to drought at the seedling stage. Studies have looked at how drought affects morphology, biochemical properties, and yield of improved soybean cultivars, but few on the below ground structures. Functional root system with a robust architectural structure gives biological advantages in case of low water availability subsequently limited nutrient uptake. Research into the impact of drought on soybean roots is required. Understanding the relationships between belowground, aboveground, and physio-biochemical drought responses will aid in cultivar improvement. Twenty distinct soybean genotypes were planted for 21 days, then 14 days of drought and normal water conditions applied. The experiment was set up in 3 blocks with 3 replicates. Above ground parameters (AGP); shoot height, number of leaves, leaf width, leaf length, canopy wilting, and shoot dry weight were measured. Below ground parameters (BGP); root length, number of lateral roots, root diameter and root dry weight were measured. Biochemical parameters (BP); Chlr a, Chlr b, carotenoids and proline contents were also measured. These parameters were compared among the 20 soybean genotypes to determine which of the cultivars possess drought-tolerance. Positive correlation exists between AGP and BGP. PCA biplot reveals genotypes TGm-1326, TGm-95, TGm-4414, TGm-1678 and TGm-99 possess strong ABG and BGP advantages under drought. For cultivars with strong BGP and BP advantages under drought, genotypes TGm-4015, TGm-4400, TGm-951, TGm-3972, TGm-4004, and TGm-4022 are the best cultivars. Soybean cultivars with advantageous BG and BP under drought will be useful in germplasm improvement.