High resolution X-ray computed tomography (HRXCT) enables sectioning-free 2D imaging of biological structures and reconstruction of 3D objects. Although its application is common in many areas of biomedicine and despite its flexibility regarding resolution levels, the technology remains underutilized in the plant sciences. Here, we established HRXCT for the study of parasitic plant-plant interactions by presenting a sectioning-free protocol to access soft-tissue host-parasite contact zones at cell level resolution. We tested various sample preparation methods and contrast stains for their efficiency to improve resolution of haustorium-host samples at the cellular level. In doing so, we achieved cellular resolution with visible cellular organization of haustorium structures, especially on the vascular system for fresh and contrast-stained samples. Fresh stained and dehydrated haustorium sample preparation enables the highest spatial resolution with a fine-cellular discrimination of haustorium vs. host cells. Application of cell-level resolved HRXCT to the pathosystems Alectra-cowpea, Phelipanche-tomato, Striga-sorghum, Phtheirospermum- tomato, and Rhamphicarpa-tomato highlighted a lifestyle-specific organization and uncovers an yet undescribed internal displacement of host tissue at parasite-host contact zones. Following image-based training, our HRXCT approach can invoke AI-based cell recognition for automated parasite cell-host cell differentiation. Superseding extensive microsectioning for 3D imaging, the newly established HRXCT protocol for 2D- and 3D- visualization of plant-plant contact zones and the first insights gained from it, is promising and useful for mid-throughput, comparative studies of parasitic plant-host interactions.