Pinguicula L. (Lentibulariaceae) is a genus of carnivorous plants known as the butterworts. Members of the genus forms a rosette of carnivorous leaves with mucilage glands from which the floral scape arises and produces one to multiple bilabiate, tubular flowers. Three subgenera comprise Pinguicula (Isoloba, Temnoceras, and Pinguicula) and 115 species are currently recognized [1, 2]. Morphological characters vary greatly among species and were once used to assign taxonomic groupings. Molecular marker-based phylogenetic reconstruction have refined Pinguiculamorphological-based taxonomy, however, infrageneric classification remains incongruent among different markers, especially for subgenus Temnoceras sect. Temnoceras [3, 4]. Meanwhile, this subgenus also displays great diversity in floral morphology. To resolve the tangled relationship, 34 representative species from section Temnoceras and section Isoloba for phylogenetic reconstruction were selected for Illumina whole genome sequencing (30Gb for each species) and assembled with MaSuRCA. Following assembly, genome completeness was estimated using BUSCO based on the eudicots_odb10 database [5]. All assemblies were found to be above 88% completeness. The phylogenetic reconstruction was generated using ASTRAL, a coalescence-based approach that incorporated over 2,000 individual gene (BUSCOs) trees into species tree estimation [6]. A robust phylogeny has been reconstructed, with posterior probabilities high for most branches (1). Pinguicula morphological characters have also been comprehensively collected from publications [7]. The phylogenetic reconstruction will serve as the basis for following comparative phylogenetic methods and statistical analyses to investigate Pinguicula infrageneric classification and phenotypic trait evolution.  Reference: Barnhart, J. H. (1916). Segregation of genera in Lentibulariaceae. Mem. NY Bot. Gard., 6, 39-64. Fleischmann, Andreas. (2021). On the infrageneric classification of Pinguicula. Carnivorous Plant Newsletter. 50. 174-188. 10.55360/cpn504.af487. Fleischmann A, Roccia A. Systematics and evolution of Lentibulariaceae: I. Pinguicula. In: Elison AM, Adamec L, editors. Carnivorous plants, physiology, ecology, and evolution. Oxford: Oxford University Press; 2018. pp. 70–80. Shimai, H., Setoguchi, H., Roberts, D. L., & Sun, M. (2021). Biogeographical patterns and speciation of the genus Pinguicula (Lentibulariaceae) inferred by phylogenetic analyses. PloS one, 16(6), e0252581. Waterhouse, R. M., Seppey, M., Simão, F. A., Manni, M., Ioannidis, P., Klioutchnikov, G., ... & Zdobnov, E. M. (2018). BUSCO applications from quality assessments to gene prediction and phylogenomics. Molecular biology and evolution, 35(3), 543-548. Mirarab, S. et al. ASTRAL: genome-scale coalescent-based species tree estimation. Bioinformatics 30, i541-i548 (2014). Lampard, Stan & Gluch, Oliver & Robinson, Alastair & Fleischmann, Andreas & Temple, Paul & McPherson, Stewart & Roccia, Aymeric & Partrat, Eric & Legendre, Laurent. (2016). Pinguicula of Latin America.