Legumes are heavily dependent upon the activities of microorganisms such as bacteria, archaea, and fungi to overcome ecological stressors. Immediate soil environments hold a cornucopia of microorganisms for legumes to select for plant microbiome colonization (Brown, Grillo et al. 2020). The composition of the soil microbiome is dependent upon a series of plant-soil feedbacks between legumes and soil microbial in which specific microbial taxa within the soil are enhanced through excretion of a series of photosynthetically-derived exudates through legume roots. From these enriched communities, plants can select and acquire microbes that mediate plant functional traits such as nutrient acquisition, abiotic stress tolerance, and pathogen and parasite resistance (reviewed in Friesen et al. 2012; Gutierrez & Grillo 2022). The enrichment of rhizobial bacteria has consistently been a taxa of interest within legume-soil feedback systems due to their ability to infect nodules, a distinct plant compartment in which rhizobia can fixate nitrogen into usable forms for legume growth and development. However, the impact of plant-soil feedback on the structure of larger soil microbiomes is largely unknown. To elucidate further the relationship of rhizodeposition and the structure of soil microbial communities seven different legume species will be grown in rhizosphere soils collected from the Chicago region, soils collected ~100 yards from rhizosphere soil collections, and a common garden soil to represent soil communities at vary levels of enrichment prior to planting. 16S amplicon sequencing will be used to characterize the soil communities at varying stages before and after enrichment.