The Old World Climbing fern, Lygodium microphyllum, is a highly successful invasive species in the southeastern United States. Native to much of eastern Asia, the Pacific Islands, and Africa, introduction of the species to Florida in the late 1800s through the cultivated plant trade has led to rapid population establishment, growth, and spread in the state over the past four decades. Lygodium microphyllum forms thick mats that smother and shade native plants and vulnerable ecosystems with fronds that can reach up to 30m in length by indeterminant growth. Herbicide and biological control measures have been largely unsuccessful at mitigating the spread of this fern. Here, we report on a preliminary assembly of the genome of L. microphyllum using a combination of long- and short-read sequencing technologies. Using this new genomic resource, we aim to explore functional differences between the gametophyte and sporophyte life stages through multiple facets including physiological, transcriptomic, and epigenomic avenues. We hope the integration of these genomic tools with applied sciences will yield downstream applications to aid in the prevention of further spread and containment of Lygodium microphyllum.