Poster

         Comparative Genomics/Transcriptomics

A high-quality nuclear genome of the carnivorous plant Triantha occidentalis (Tofieldiaceae)

Presenting Author
Philippa Stone
Description
Triantha occidentalis (Tofieldiaceae, Alismatales) is a semi-aquatic, perennial, rhizomatous herb found along the west coast of North America from Alaska to California. Triantha occidentalis was recently discovered to be carnivorous, catching insects using a sticky trap directly below its inflorescence. Triantha occidentalis is of particular interest as it is one of only five species of carnivorous monocot, representing four independent evolutions of carnivory within the clade. We confirmed the karyotype of T. occidentalis to be 2n = 15 using chromosome squashes, and estimated its genome size to be 1C = 2.3Gb using flow cytometry. We sequenced the nuclear genome of T. occidentalis using PacBio HiFi technology and assembled it using Hifiasm. Our combined-haplotype assembly for the nuclear genome of T. occidentalis is over 2.1 Gb long, has a contig N50 of over 42 Mb, and a GC content of 43.2%. The current assembly has a complete BUSCO score of 98.2% and only 0.8% of BUSCOs are missing. We are currently sequencing a chromosomal confirmation capture (Hi-C) library to improve our assembly, and are annotating the genome using the BRAKER pipeline supported by RNA-seq data from the leaves, sticky trap, and inflorescence of the plant. We will use this later to explore gene expression differences that may have consequences for our understanding of the plant’s ecology, evolution and function.