The tribe Lithospermeae (Boraginaceae) includes ca. 450 species, with most distributed across the Old World.  The tribe houses a variety of notable speciose genera, including Onosma, Lithospermum, and Echium, as well as a variety of genera with fewer than 10 species.  During the past 15 years, the tribe has been the subject of multiple phylogenetic studies, with most including only a few DNA regions.  To better understand the evolutionary relationships within the tribe, we employed genome skimming to assemble the plastid genome and nuclear ribosomal cistron and reconstructed phylogenies from these genomic regions.  Additionally, we investigated patterns of evolution and selection across the plastid genome.  The resulting phylogenies provide evidence that, in general, relationships across the tribe are similar to those that have been previously resolved; however, the addition of greater sequence data suggests that some genera, such as Alkanna, are resolved as non-monophyletic, and other genera, such as Onosma, that were sometimes recovered as paraphyletic may, indeed, be monophyletic.  Similar results are reconstructed among the two genomes.  For the plastid genome, genes at the IR boundary are fairly conserved across most of the tree, but among Onosma and relatives, greater variation is identified.  Most areas of the plastid genome are not under positive selection, but five genes are recognized as being under positive selection.