Carbon (C), nitrogen (N) and phosphorous (P) affect plant function, growth, distribution, and biogeochemical cycles in which plants participate. Due to isotopic differences between different plant tissues, the CNP isotope composition of plants can comprehensively reflect information on climate change and ecosystems’ nitrogen cycle under different biotic and abiotic stress conditions. Plant isotopes can also help reveal how species adjust resource acquisition strategies and life history strategies to ensure improved competitiveness and viability. In this study, we measured the δ 14C, δ 15N and δ 33 P values (δCNP) of seedlings of eight Ficus species, that were grown in germination chambers, which were subjected to a range of N and P addition treatments during early regeneration. We hypothesized that the four species of non-hemiepiphytic Ficus species will have greater (δCNP) values compared to the congeneric hemiepiphytic species and that the (δCNP) will be positively correlated with greater addition of P&N to the growth media. These differences may provide important insights into the use of CNP isotope techniques to identify the changes that occur when plants in different growth forms face environmental changes or stress.