Nectary function is central to many important ecological interactions. Across flowering plants, nectaries are diverse in positional location and morphology, yet few have used genomic approaches to better understand how diverse nectaries develop. Monocot flowers usually form septal nectaries, which are found on the margins between the three fused carpels that comprise the gynoecium. While key transcription factors underlying nectary development in eudictos have been characterized, the genetic basis of nectary development in monocots is virtually unknown. As a first step to identify candidate genes involved in monocot nectary development, we are using single nuclei RNA sequencing. We isolated and sequenced banana (Musa “Ice Cream”) nuclei from two sets of tissues—one region that is enriched in nectary cells found at the top of gynoecium (the “nectary”) and the other that primarily lacks nectary cells found at the base of the gynoecium (the “base”). We compare results from banana with nuclei sequences from asparagus (Asparagus officinalis) to determine whether putative nectary cell clusters from both species share a common core set of genes for nectary development. Results from this study will form the foundation for understanding nectary origins and losses in monocots and more broadly across flowering plants.