Plant sexual reproduction strategies can be represented by a continuum from outcrossing, where two genetically distinct plants contribute hereditary material to offspring, and selfing, where the entirerity of the offspring’s genetic information comes from a single parent. Plant species can be placed on this spectrum according to the degree to which they employ either strategy for sexual reproduction. In mixed-breeding species, those that utilize both outcrossing and selfing, the genetic diversity of populations will differ based on the degree to which either strategy is used for seed production. Therefore, by examining the genetic structure of mixed-breeding populations, plant breeding systems can be estimated and placed on this continuum. The genus Viola is well known for its exhibition of cleistogamy, an extreme form of selfing characterized by the formation of closed, budlike flowers. In addition, violets also produce chasmogamous flowers, most of which can outcross and self. However, the extent to which these floral form contribute to the establishment and growth of populations is understudied and unknown in the taxonomically complex high polyploid stemless blue violet lineage. The present study seeks to address these questions by examining the population genetics of four species of stemless blue violets and comparing them to the exclusively chasmogamous and self-incompatible V. pedatae. Five individuals from a total of 15 populations were sampled and submitted for genotyping-by-sequencing. SNPs were called with iPyRad, as this method incorporates ploidy information. Population genetic parameters were estimated with GenoDrive and patterns of population structure were analyzed with STRCUTURE and DAPC. From the results of this study, we aim to evaluate how chasmogamous and cleistogamous flowers contribute to seedling recruitment and differ among species and populations.