In Hmong culture it is tradition to purify the body for 30 days with a chicken soup diet after giving birth. Recent work to provide culturally equitable healthcare has led hospitals in the Twin Cities, MN, where there is a large Hmong community, to offer the postpartum chicken soup to new mothers. We worked with local Hmong farmers and stewards of their family chicken soup recipe, Zongxee and Mayyia Lee, to identify the medicinal herbs used in postpartum chicken soup using the internal transcribed spacer (ITS) region and sequencing the DNA after PCR. PCR results were analyzed using Geneious. The resulting sequences were compared against sequences in GenBank using BLAST and phylogenetic reconstruction. The experiment was designed to improve medicinal herb research and knowledge for the benefit of postpartum recovery patients. The results will provide a foundation for evidence based research on medicinal herbs and be returned back to the Hmong community to help preserve cultural knowledge.