Hosting over 4,200 species of vascular plants, Florida overlaps two globally recognized biodiversity hotspots, the North American Coastal Plain (NACP) and the Caribbean Islands. Unfortunately, habitat destruction and climate change pose serious threats to the maintenance of this floristic diversity. Quantifying biodiversity for conservation purposes has typically relied on methods that evaluate Species Richness (SR), which does not incorporate the evolutionary history of the community, and how this may influence the community assembly. The massive amount of digitized herbarium specimen records available through data aggregators like iDigBio, GBIF, and SERNEC, facilitate the aggregation of records representing species in the vascular flora of Florida. The goal of my research is to locate current concentrations of significant biodiversity, using a phylogeny representing 99% of the vascular flora of Florida. This phylogeny was generated by generating an alignment using MAFFT using open source DNA sequences from GenBank representing the rbcL and matK gene regions of the chloroplast. These two alignments were concatenated and used to generate a phylogeny using RAxML. With this phylogeny, I plan to use spatial phylogenetic metrics, such as Phylogenetic Diversity (PD),  to assess the diversity of plant assemblages with an evolutionary context. Other metrics such as Phylogenetic Endemism (PE), Relative PD (RPD), Relative Phylogenetic Endemism (RPE), and Categorical Analyses of Neo- and Paleo- Endemism (CANAPE). The overlap between currently conserved land in Florida and areas of significantly high PD patterns will be assessed. These analyses will provide a more thorough understanding of the spatial phylogenetic diversity patterns in Florida. Here we present results from a phylogeny representing 99% of the vascular plants of Florida. This phylogeny can be used to analyze various spatial phylogenetic patterns in Florida and make recommendations for conservation efforts and land managers for bettering the conservation of Florida's evolutionary biodiversity hotspots.