Plants have evolved different mechanisms to attract pollinators and promote outcrossing. One of these mechanisms is the production of floral nectar. Nectar is a sugary substance that plants produce to attract pollinators to visit their flowers. This sweet liquid is composed of mainly carbohydrates, water, amino acids, and secondary metabolites. When analyzed, the composition of nectar follows specific trends. The nectar of a plant that is primarily pollinated by a hummingbird is very different than that of a plant that is pollinated by a bee or hawkmoth. For example, the nectar of a plant that is pollinated by hawkmoths has a higher sucrose concentration than a plant pollinated by bees or hummingbirds. The genetic intricacies that control this phenomenon are not well defined. Currently, the literature on the genetic control of nectar composition in plants is very limited. Most of the work done has concentrated on isolating specific genes involved in nectar production. Very few studies have looked at entire genetic pathways involved in nectar production and secretion. Here, I will use comparative transcriptomics to identify the genes that influence nectar composition across related species of Aquilegia with different animal pollinators. To determine which genes are associated with variation in nectar composition between species with different animal pollinators, I will examine gene expression in nectary tissue from four species of Aquilegia, including a primarily bee-pollinated species, two primarily hummingbird pollinated species, and a hawkmoth-pollinated species at different developmental stages. I will then identify genes that are differentially expressed by using Illumina sequencing to compare the RNA present in the nectary at the point of collection. I intend to then use Gene ontology enrichment analysis to define the identity or functions of expressed orthologs in other species.