Orchids are facing global threats and extinctions at an alarming rate. Florida is a hotspot for native orchid biodiversity; making up at least half of the species diversity found in continental United States and are mainly distributed in the Everglades and Fakahatchee Strand. Anthropogenic activity has had significant negative impacts on orchid biodiversity in the state such as; habitat loss, climate change, and illegal collection. It is estimated that roughly 50% of Florida natives are listed as threatened, endangered, or critically imperiled. The Pine-Pink orchid, Bletia purpurea is a state-threatened terrestrial species that was historically abundant throughout South Florida. Urbanization and development of the Greater Everglades ecosystem over the last 200 years have left populations of B. purpurea reduced and fragmented overtime. As a space-dependent species, they can be locally abundant but only occur in a limited number of sites. Dispersal may be restricted due to natural factors or anthropogenic activities, and may be reproductively isolated. Local adaptations have been observed between the east and west coasts of South Florida; such as differences in habitat preference and blooming periods. Bletia purpurea in Florida has developed self-fertilization, a form of autogamy, which is not commonly reported in their Caribbean and Latin counterparts. It is becoming increasingly clear that genetic information is critical for forming conservation strategies to help mitigate the disappearance of threatened species from the wild. Conservation research scientists from the Atlanta Botanical Garden designed and developed a lineage-specific target sequence capture bait set for the Orchidaceae family to reliably sequence orthologous loci across many orchid lineages. This bait set has not yet been tested on any species from the genus Bletia, but shows great promise for estimating population genetic diversity and structure, especially within the Epidendroideae subfamily. To date, there is nothing known about the historic or current population structures of Bletia purpurea or its intraspecific genetic diversity among the known distribution areas in Florida. This study aims to focus on their distribution throughout Palm Beach, Miami-Dade, and Collier County. Due to the cleistogamous nature of this species in Florida and the large physical distances from each area (>100km), it is hypothesized that each region will be genetically distinct with high variation between populations.