Salicornia are globally-distributed halophyte plants that thrive in the high intertidal zone and colonize salt marsh mudflats in the first stage of succession. Salicornia hyperaccumulate metals by stabilizing them in plant tissue, potentially providing remediation services. Salicornia also can be eaten as a vegetable and used for seed oil and “green salt” in emerging industries. Most research in this genus has focused on European species and comparatively little is known about North American species, including the widely-distributed S. depressa. No Salicornia genome resources are currently available. We are assembling the S. depressa genome using a combination of long read sequencing, Hi-C mapping, and short-read polishing, and annotating the genome with transcriptome and methylome data. Our initial genome profiling indicates that S. depressa is a diploid with a genome length of approximately 1.13 Gbp and 6.87% heterozygosity, contradicting the literature reporting the species as tetraploid and primarily selfing. We confirmed our results with additional sequencing and root-tip chromosome squashes (2N = 18). We have also obtained low-coverage whole genome sequence data from 94 individuals from ten populations sampled across New England at sites ranging from pristine to metal-contaminated. Our preliminary analyses of genomic and population structure in S. depressa provide insight into the evolutionary history of this understudied species and will support future research on salinity and metal tolerance.