Tennessee and Kentucky are centrally positioned in the interior Southeastern US and combined they contain ~3600 vascular plant species and lesser taxa. There are ~900 additional species or lesser taxa across the broader region around these two states, and these have been included to ensure inclusion of all potential species in Tennessee and Kentucky. Launched in 2020, the Tennessee-Kentucky Plant Atlas (TNKY) has become a major resource for botanists, conservation workers, students, and teachers in the region. The TNKY Atlas is one of a kind, allowing for individuals to search not just by taxonomy, but by any one of 205 characters in the database. Underlying TNKY is a database of more than 200 taxonomic, conservation, ecological, morphological, and reproductive characters for the ~4500 taxa. In total, this makes for a database of about one million cells. While a work in progress, here we report on some characteristics of the flora, which is represented by 215 families, 1024 genera, and ~4500 species and lesser taxa. Ninety-five percent of species are angiosperms, with only 3% pteridophytes and less than 1% gymnosperms. Conservation characteristics include nativity, region of origin, invasiveness, global and state ranks, federal listings, and wetland statuses. Ecological characters range from general ecological habitats of terrestrial or aquatic, soil and moisture regimes, to level three and four ecoregions. The database has more than 20 characters related to leaf and stem morphology and about 30 reproductive characteristics. Eighty percent of the flora is native and introduced species mainly originate from Europe and Asia. Over 250 species are listed as invasive by at least one southeastern state. These invasive species tend to follow the general trends of the database. This regional flora contains sixty-seven G1 species and 36 federally listed species. Eighty-seven percent of all species are terrestrial, 7% are in mudflats and wet woods, 3% are aquatic, and 1% are strict wetland species. Fifty-six percent are forbs, 19% are graminoids, and 17% are woody. Seventy-six percent are perennials, 19% are annuals, and 4% are biennials. Fifty-two percent have alternate phyllotaxy, 26% have basal phyllotaxy, 17% have opposite phyllotaxy, and 3% have whorled phyllotaxy. Eighty-four percent have simple leaves, 15% have compound leaves, and less than 1% have absent leaves or are thalloid. The majority of species reproduce in May, June, July, or August, with 30 species reproducing in all 12 months. Research is ongoing and will continue to fill in more characteristics present within the database, hopefully with a focus on rare species.