We present on progress in developing a DNA barcode reference library for the bryophyte flora of British Columbia (BC). DNA barcoding is a method used to quickly identify species based on standardized molecular markers. Ideally, a barcode sequence is highly similar in all conspecifics and distinct from close relatives. The technique is useful for accurately identifying cryptic taxa or fragmentary samples, where morphology-based identifications may not be conclusive. Bryophytes can be more difficult to identify morphologically than vascular plants and may be underrepresented in regional floristic studies. As such, there exists a need to produce high-quality voucher-backed DNA barcode reference libraries to enable efficient identification and surveying of bryophyte taxa, particularly in British Columbia (BC) within Canada, which possesses the richest bryophyte diversity of any province. We assess sequence recoverability for common barcode markers and compare marker effectiveness in identifying individual species. Our reference library provides a useful resource for metagenomic and ecological genetic research in BC, and more broadly in North America and other temperate regions, and so will also contribute to national and international barcoding efforts.