As humans redistribute species across the globe and our climate changes at an unprecedented rate, it is urgent that we cultivate a robust understanding of the underlying mechanisms for colonization success, such as phenotypic plasticity. Phenotypic plasticity is the ability for a single genotype to differentially express alternative phenotypes based on the environment. By capitalizing on the abundant genomic and germplasm resources of the globally distributed species, Arabidopsis thaliana, we implemented a full-factorial design to test whether populations demonstrating recent successful colonization are more plastic. We selected twenty-two populations from A. thaliana’s global range that represent the following groups based on their genomic cluster identities: “European colonizers”, “North American colonizers”, and “relicts”. Using these group classifications, we are testing if plants that have a recent history of colonization (the North American colonizers) are more plastic than those in the native range but have expanded into available habitats (European Colonizers) and those that have remained in their glacial refugia (Relicts). Trait plasticity was quantified using the F-ratios generated from the following model: trait ~ treatment. We used a linear mixed effects model to empirically test for significant F-ratio differences between our groups of interest. The results of our study improve our understanding of phenotypic plasticity’s role in plant colonization. This knowledge will enable us to forecast the changes we may see in our flora and ultimately, Earth’s ecosystems.