Crops often undergo flooding stress, thus reducing the amount of oxygen available for respiration within roots. Flooding-tolerant plants have the ability to produce aerenchyma, which occurs as large intercellular air spaces in root cortex to allow for aeration of submerged tissues. It was hypothesized that winter wheat under flooding conditions would grow a greater amount of aerenchyma than wheat under drained conditions. To investigate plant responses, winter wheat was grown in flooding and drained conditions, with roots harvested at 0, 1, 3, 7, 14, and 21 days of treatment. When assessed microscopically, root sections of flooded plants had more aerenchyma than roots in drained conditions. Aerenchyma development increased over time, appearing around 3 days of flooding and reaching a maximum between 7 and 14 days of treatment. Air spaces in aerenchyma function to ventilate root systems, allowing the plants to tolerate hypoxic soil conditions typical of flooding. Flooded conditions were correlated with an increased amount of aerenchyma in winter wheat. Future work repeating these methods with other crop species could be beneficial. This work would allow for informed agricultural decisions to be made, regarding the selection of crops best fit for environmental conditions.